Molecular Longevity
Lab Members
Lab Resources
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Protocols
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Yeast
C. elegans
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Rotifers
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Molecular Longevity Protocols
Media Recipes
Luria Broth (LB)
YPD Media
Basic Media (Yeast)
Synthetic Complete (and drop-out) Media
40% glucose
C. elegans
NGM
M9
22ppt Seawater and Algae (Rotifers)
E. coli
Protocols
E. coli
transformation
Freezing bacterial stocks
Yeast Protocols
Yeast Glycerol Stocks
Easy Yeast DNA Extraction
High Efficiency Yeast Transformation (for integration)
One-step Transformation (for plasmid transformation)
Yeast DAPI Staining
C. elegans
Protocols
RNA extraction
C. elegans
DAPI staining
Freezing Down
C. elegans
Stocks (and Soft Agar Solution)
Rotifer Protocols
Amictic egg harvest
Tissue Culture Protocols
Cell Culture Passaging
Hemocytometer (Cell Counting)
siRNA Transfection of 3T3 Cells
RNA Extraction from Tissue Culture Cells
Molecular Biology Protocols
Agarose Gel Electrophoresis (TBE and 1kb Ladder Recipes)
Polymerase Chain Reaction (PCR)
Molecular Cloning
USER Cloning
cDNA Synthesis (Superscript IV First Strand Synthesis)
Western Blot
Miscellaneous Protocols
Agarose pads for microscopy